Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) from Abcam

Product Specs
ItemMitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab110171
Quantity1 kit
TargetMitochondria Isolation

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defectsAging cellOctober 1, 2023Oleinik N, Albayram O, Kassir MF, Atilgan FC, Walton C, Karakaya E, Kurtz J, Alekseyenko A, Alsudani H, Sheridan M, et al.(Oleinik et al., 2019). 4.6 Cell fractionation For mitochondria preparation, Mitochondria Isolation Kit (ab110171, Abcam) was used according to manufacturer instructions. Mitochondria were isolated, and submitochondrial

(2) Protease-independent control of parthanatos by HtrA2/OmiCellular and molecular life sciences : CMLSAugust 18, 2023Weiß J, Heib M, Korn T, Hoyer J, Fuchslocher Chico J, Voigt S, Koudelka T, Tholey A, Adam D.biological triplicates with the Mitochondria Isolation Kit for Cultured Cells with Dounce homogenizer (ab110171, Abcam) following the instructions of the manufacturer. To 200 µg of each fraction, SDS was added [1%

(3) Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesisNature immunologyJune 1, 2023Yazicioglu YF, Marin E, Sandhu C, Galiani S, Raza IGA, Ali M, Kronsteiner B, Compeer EB, Attar M, Dunachie SJ, et al.°C). Cells were then homogenized with a Dounce homogenizer with a 2-ml reservoir capacity (catalog no. ab110171, Abcam), using ten strokes with a type B pretzel. The homogenizer was rinsed with distilled water before

(4) TIMP-1 Dependent Modulation of Metabolic Profiles Impacts Chemoresistance in NSCLCCellsSeptember 28, 2022Wei Xiao, Pankaj Ahluwalia, Lan Wang, John Howard, Ravindra Kolhe, Amyn M. Rojiani, Mumtaz V. RojianiMitochondria Isolation The mitochondrial fraction was isolated using Mitochondria Isolation Kit for Cultured Cells (ab110171, Abcam, Boston, MA, USA) according to the manufacturer’s instructions. Briefly, NSCLC cells were washed

(5) Systematic evaluation of parameters in RNA bisulfite sequencing data generation and analysisNAR Genomics and BioinformaticsJune 3, 2022Zachary Johnson, Xiguang Xu, Christina Pacholec, Hehuang Xielibrary construction Mitochondria were isolated from NSCs using a mitochondrial isolation kit (Abcam, ab110171) following the manufacturer's instructions. RNA was extracted from the isolated mitochondria and subjected

(6) Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosisFree radical biology & medicineDecember 1, 2020Takashi Y, Tomita K, Kuwahara Y, Roudkenar MH, Roushandeh AM, Igarashi K, Nagasawa T, Nishitani Y, Sato T.Mitochondria were isolated from WI-38 cells (RIKEN BRC, Ibaraki Japan) using a mitochondrial isolation kit (ab110171, Abcam, Cambridge, UK) for 24 h, as described previously [30]. Then, transferred-mitochondria (Mito)

(7) BRCA1 Deficiency Impairs Mitophagy and Promotes Inflammasome Activation and Mammary Tumor MetastasisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2020Chen Q, Lei JH, Bao J, Wang H, Hao W, Li L, Peng C, Masuda T, Miao K, Xu J, et al.isolation were performed by Mitochondria Isolation Kit according to the manufacturer's instructions (Abcam, ab110171). For MCF7 cells, isolated mitochondria were treated by proteinase K (0.8 mg mL−1) alone or proteinase K

(8) Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT)PloS oneJanuary 1, 2020Nakano D, Kawaguchi T, Iwamoto H, Hayakawa M, Koga H, Torimura T.fraction Mitochondrial fraction was isolated by using Mitochondria Isolation Kit for Cultured Cells (ab110171, Abcam) according to the manufactures’ instruction. Briefly, Hep3B and Huh7 cells were collected with

(9) Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPKCell ReportsNovember 5, 2019Yu Wang et al.Assays,Critical Commercial Assays Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171),Abcam,Cat# ab110171 TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852),Abcam,Cat# ab113852 MitoTox Complex I OXPHOS Activity Assay Kit

(10) Mitochondrial protein import is regulated by p17/PERMIT to mediate lipid metabolism and cellular stressScience advancesSeptember 1, 2019Oleinik N, Kim J, Roth BM, Selvam SP, Gooz M, Johnson RH, Lemasters JJ, Ogretmen B.1010 TEM. Subcellular fractionation For mitochondria preparation, the Mitochondria Isolation Kit (ab110171; Abcam) was used, according to the manufacturer’s instructions. Briefly, trypsinized cells were collected

(11) Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancerProceedings of the National Academy of SciencesFebruary 20, 2018Felipe Paredes et al.commercial kit (ab83431; Abcam). PDH and α-KGDH activities were determined in isolated mitochondria (ab110171; Abcam) using colorimetric enzymatic activity assays (MAK183 and MAK189, respectively; Sigma). Statistical

(12) Mitochondrial Complex I Reversible S-Nitrosation Improves Bioenergetics and Is Protective in Parkinson's DiseaseAntioxidants & redox signalingJanuary 1, 2018Milanese C, Tapias V, Gabriels S, Cerri S, Levandis G, Blandini F, Tresini M, Shiva S, Greenamyre JT, Gladwin MT, et al.cells were isolated according to the ‘‘Mitochondria Isolation Kit for Cultured Cells’’ guidelines (ab110171; Abcam, Cambridge, MA), with minor modifications. Briefly, 4 · 108 confluent cells were collected by

(13) TNF-α promotes extracellular vesicle release in mouse astrocytes through glutaminaseJournal of neuroinflammationApril 20, 2017Wang K, Ye L, Lu H, Chen H, Zhang Y, Huang Y, Zheng JC.Substrate. Mitochondrial protein was isolated by the Mitochondria Isolation Kit for Cultured Cells (ab110171, Abcam). ATP5A (1:1000; Abcam) was used as a mitochondrial marker to indicate that there is no mitochondria

(14) Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetesRedox biologyApril 1, 2017Gómez-Serrano M, Camafeita E, López JA, Rubio MA, Bretón I, García-Consuegra I, García-Santos E, Lago J, Sánchez-Pernaute A, Torres A, et al.Mitochondria were obtained from mature frozen adipocytes using the Mitochondria Isolation Kit (Cat. ab110171, Abcam), according to the manufacturer's instructions. Concentration of mitochondrial suspension was

(15) Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1EMBO molecular medicineJune 1, 2015Seillier M, Pouyet L, N'Guessan P, Nollet M, Capo F, Guillaumond F, Peyta L, Dumas JF, Varrault A, Bertrand G, et al.according to the manufacturer's instructions of Mitochondria Isolation Kit for Cultured Cells (Abcam #ab110171, Paris, France). Briefly, after one freeze–thaw cycle, pellets from 2 dishes were resuspended in 750

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Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) from Abcam

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Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) from Abcam

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